3 billion years Nitrogenase history on predicted structures
Update: The article was published on eLife
Last week, we released our latest pre-print, available here. Our study aims to understand the evolutionary history of Nitrogenase over the past 3 billion years. We employed a combined approach involving ancestral sequence reconstruction, AlphaFold-based protein structure prediction, and structural characterization.
This publication introduces a new feature I developed: a Streamlit-based web server that provides access to all our predictions. The server is hosted at nsdb.bact.wisc.edu and offers an intuitive interface to explore the temporal evolution and structures of Nitrogenases. Implementing this server was challenging, primarily due to the stringent security requirements for deploying a URL under the wisc.edu domain.
As the paper is currently a pre-print, I would greatly appreciate any comments or feedback you might have on our work.